Journal: PLoS ONE

Article Title: Regeneration of esophagus using a scaffold-free biomimetic structure created with bio-three-dimensional printing

doi: 10.1371/journal.pone.0211339

Figure Lengend Snippet: The bio-3D printed structures are shown before transplantation. A-D: HE staining. E-H: Immunohistochemical staining with anti-αSMA antibodies. I-L: Immunohistochemical staining with anti-VEGF antibodies. M-P: immuno-histochemical staining with anti-CD31 antibodies. Q-T: Immunohistochemical staining with anti-elastin antibodies. Scale bar = 100 μm. HE: hematoxylin-eosin, SMA: smooth muscle actin, VEGF: vascular endothelial growth factor.

Article Snippet: Immunohistochemistry was performed with the following primary antibodies: alpha smooth muscle antibody (αSMA; 1:200; mouse monoclonal; A5228; SIGMA-ALDRICH, St. Louis, MO, USA) for evaluation of the smooth muscle cell distribution, anti-CD31 (1:400; rabbit polyclonal; bs-195R; Bioss, Boston, MA, USA) for evaluation of the endothelial cell distribution, anti-vascular endothelial growth factor (VEGF; 1:200; rabbit polyclonal; sc-152; Santa Cruz, Texas, USA) for evaluation of vasculogenesis, elastin (1:200; rabbit polyclonal; ab21610; Abcam, Cambridge, UK) for evaluation of generation of ECM, anti-pan-cytokeratin (1:200, mouse monoclonal; ab7753; Abcam) for evaluation of epithelialization, and anti-HLA class I ABC antibody (1:20, mouse monoclonal; ab70328; Abcam) for evaluation of distribution of implanted structure made of human cells.

Techniques: Transplantation Assay, Staining, Immunohistochemical staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: Biomarkers employed for identification of VM versus true angiogenesis.

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques:

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: WaGa and MKL-1-derived xenograft tumors contained CD31 − anastomosing networks that were enhanced with PAS staining, and were associated with staining for laminin (Lam), CD144, Nodal and VEGFR-1. Note the density and complexity of laminin + structures in comparison to CD31 + vessels (CD31 and Lam panels original magnification, 200×; PAS, CD144, Nodal, and VEGFR-1 original magnification, 1000×).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Derivative Assay, Staining

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: MKL-1 tumors treated with vehicle, carboplatin and etoposide stained for CD31 (upper panels; original magnification, 100×) and laminin (Lam) (middle panels; original magnification, 100×); rectangular regions are representative fields enlarged for clarity (original magnification, 1000×). Note the marked and widespread increase in laminin + networks after chemotherapy. A similar picture is observed in WaGa derived tumors, although less diffusely (data not shown). A statistically significant increase in laminin immunoreactivity after carboplatin is observed in both cell lines, and after etoposide in MKL-1 derived tumors (graphs, lower panels).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Staining, Derivative Assay

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: Relative LAMA3 (left), LAMB3 (center, left), LAMC2 (center, right) and CD144 (right) mRNA expression (mean ± s.e.m.) by carboplatin- and etoposide-resistant versus vehicle-treated MKL-1 (top) and WaGa (bottom) cells, as determined by real-time quantitative RT-PCR. Established human umbilical vein endothelial cells (HUVEC) served as a positive control. Data are representative of n=3 independent experiments. P<0.05, ** P<0.01, *** P<0.001).

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Expressing, Quantitative RT-PCR, Positive Control

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Merkel Cell Carcinoma Expresses Vasculogenic Mimicry: Demonstration in Patients and Experimental Manipulation in Xenografts

doi: 10.1038/labinvest.2014.99

Figure Lengend Snippet: PAS-positive networks also are demonstrable by laminin IHC, and a minority of these are associated with CD31 staining in adjacent sections original magnification, 100×; inset, 1000×). Double IF labeling for CD31 and CD144 demonstrates an architectural pattern of CD144 positivity similar to that seen with laminin, and distinct from the comparatively few and discrete CD31 + vessels (original magnification, 200×)

Article Snippet: All sections were incubated overnight with primary antibodies at room temperature (~25°C) followed by 2-hour incubation with HRP-conjugated goat anti-rabbit (for Ulex, human and mouse CD31, CD144 and laminin), horse anti-goat (for VEGFR-1) and horse anti-mouse (for Nodal) secondary antibodies (all Vector Laboratories, Burlingame, CA) at room temperature.

Techniques: Staining, Labeling